Single- and multi-photon excited fluorescence from serotonin complexed with B-cyclodextrin
Botchway, SW, Bisby, RH, Dad, S and Parker, AW 2006, 'Single- and multi-photon excited fluorescence from serotonin complexed with B-cyclodextrin' , Photochemical and Photobiological Science, 5 , pp. 122-126.
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The fluorescence of serotonin on binding with B-cyclodextrin has been studied using both steady-state and time-resolved methods. Steady state fluorescence intensity of serotonin at 340 nm showed ~ 30% increase in intensity on binding with Ka ~ 60 dm3 mol 1 and the fluorescence lifetimes showed a corresponding increase. In contrast, the characteristic green fluorescence (‘hyperluminescence’) of serotonin observed upon multiphoton near-infrared excitation with sub-picosecond pulses was resolved into two lifetime components assigned to free and bound serotonin. The results are of interest in relation to selective imaging and detection of serotonin using the unusual hyperluminescence emission and in respect to recent determinations of serotonin by capillary electrophoresis in the presence of cyclodextrin. The results also suggest that hyperluminescence occurs from multiphoton excitation of a single isolated serotonin molecule.
|Uncontrolled Keywords:||serotonin, cyclodextrin, fluorescence lifetime, multiphoton, hyperluminescence|
|Themes:||Subjects / Themes > Q Science > QH Natural history > QH301 Biology|
Subjects outside of the University Themes
|Schools:||Colleges and Schools > College of Science & Technology|
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
|Journal or Publication Title:||Photochemical and Photobiological Science|
|Publisher:||Royal Society of Chemistry|
|Funders:||Science and Technology Facilities Council (STFC), Biotechnology and Biosciences Sciences Research Council (BBSRC)|
|Depositing User:||RH Bisby|
|Date Deposited:||20 Sep 2010 10:21|
|Last Modified:||17 Feb 2014 15:46|
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