Skip to the content

An-harmonic behaviour in the multi-subunit protein apoferritin as revealed by quasi-elastic neutron scattering

Telling, M, Neylon, C, Arrighi, V and Kilcoyne, SH 2008, 'An-harmonic behaviour in the multi-subunit protein apoferritin as revealed by quasi-elastic neutron scattering' , Journal of Physical Chemistry B, 112 (35) , pp. 10873-10878.

[img] PDF - Accepted Version
Restricted to Repository staff only

Download (628kB) | Request a copy

    Abstract

    Quasi-elastic neutron scattering (QENS) has been used to study the deviation from Debye-law harmonic behaviour in lyophilized and hydrated apoferritin; a naturally occurring, multisubunit protein. While analysis of the measured mean squared displacement (msd) parameter reveals a hydration-dependent inflection above 240 K, characteristic of diffusive motion, a hydration-independent inflection is observed at 100 K. The mechanism responsible for this low temperature an-harmonic response is further investigated, via analysis of the elastic incoherent neutron scattering intensity, by applying models developed to describe side group motion in glassy polymers. Our results suggest that the deviation from harmonic behaviour is due of the onset of methyl group rotations which exhibit a broad distribution of activated processes (Ea,ave =12.2 kJ.mol-1, � = 5.0 kJ.mol�1). Our results are likened to those reported for other proteins.

    Item Type: Article
    Themes: Subjects / Themes > Q Science > QD Chemistry
    Subjects outside of the University Themes
    Schools: Colleges and Schools > College of Science & Technology
    Colleges and Schools > College of Science & Technology > School of Computing, Science and Engineering
    Colleges and Schools > College of Science & Technology > School of Computing, Science and Engineering > Materials & Physics Research Centre
    Journal or Publication Title: Journal of Physical Chemistry B
    Publisher: American Chemical Society
    Refereed: Yes
    ISSN: 1520-6106
    Depositing User: SH Kilcoyne
    Date Deposited: 22 Sep 2010 16:10
    Last Modified: 20 Aug 2013 17:25
    URI: http://usir.salford.ac.uk/id/eprint/10118

    Document Downloads

    More statistics for this item...

    Actions (login required)

    Edit record (repository staff only)