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Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan

Bisby, RH, Arvanitidis, M, Botchway, CW and Clark, IP 2003, 'Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan' , Photochemical & Photobiological Sciences, 2 (2) , pp. 157-162.

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    It is reported (Shear et al, Photochem.Photobiol. 65, 931 (1997) ) that multiphoton near infrared excitation of 5-hydroxytryptophan results in a transient product with green fluorescence. Visible fluorescence from multiphoton excitation enables detection of 5 hydroxytryptophan with extremely high sensitivity and also has potential applications in imaging of biological systems and investigation of protein dynamics. The characteristic fluorescence at 500 nm has now also been observed on one-photon photolysis of solutions at 308 nm, followed by excitation of fluorescence at 430 nm. Fluorescence was observed in aerated and deaerated solutions and in the presence of ascorbate. Enhancement of fluorescence was observed on addition of ethanol. Transient absorption experiments with 308 nm photolysis showed the formation of three transient species. In the presence of ascorbate the radical formed by photoionisation was removed, revealing a long-lived species (τ > 1 ms)) with a similar absorption spectrum, which is ascribed to the fluorescing species. Fluorescence induced by multiphoton excitation had a lifetime of 910 ± 10 ps and was also unaffected by ascorbate. In the presence of organic solvents there was an increase in fluorescence lifetime, but a decrease in overall fluorescence intensity. The fluorescence intensity and fluorescence lifetime both decreased in acidic solution (pH < 3). It is suggested that the fluorescence originates not from the 5-indoxyl radical, but from a transient product formed by intramolecular rearrangement.

    Item Type: Article
    Themes: Subjects / Themes > Q Science > QD Chemistry
    Subjects outside of the University Themes
    Schools: Colleges and Schools > College of Science & Technology
    Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
    Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
    Journal or Publication Title: Photochemical & Photobiological Sciences
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    ISSN: 1474905X
    Related URLs:
    Depositing User: H Kenna
    Date Deposited: 26 Jul 2007 16:07
    Last Modified: 20 Aug 2013 16:45

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