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Identification of cells expressing Interleukin-17A and F in the lungs of COPD patients

Eustace, A, Smyth, LJC, Mitchell, L, Williamson, K, Plumb, J and Singh, D 2010, 'Identification of cells expressing Interleukin-17A and F in the lungs of COPD patients' , Chest .

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Abstract

Background: Lymphocytes secrete IL-17A and IL-17F which enhance innate immune responses. IL-17 expression has not been studied in COPD small airways. The aim was to quantify IL-17A and IL-17F expression in the peripheral lung tissue of COPD patients compared to controls and to identify inflammatory cells that express IL-17. Methods: IL-17 expression was assessed using immunohistochemistry in peripheral lung tissue (18 COPD patients, 10 smokers and 10 non-smokers with normal lung function) and induced sputum (12 COPD patients and 6 non-smokers). Alveolar macrophages from 8 COPD patients, 8 smokers and 7 non-smokers were used for RT-PCR. Results: The numbers of inflammatory cells expressing IL-17A in the small airway subepithelium was higher in COPD patients compared to smokers (p=0.01) and non-smokers (p=0.02). IL-17A expression was higher than IL-17F in this region. IL-17A was expressed by lymphocytes, neutrophils and macrophages (confirmed by RT-PCR). The expression of IL-17F was greater than IL-17A in epithelial cells and lymphoid follicles, although there were no differences between subject groups Conclusion: Our findings indicate different roles for IL-17A and IL-17F in the pathogenesis of COPD; IL-17A plays a role in small airway subepithelial inflammation, while IL-17F appears to play a more prominent role within lymphoid follicles.

Item Type: Article
Themes: Subjects / Themes > Q Science > QR Microbiology
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Journal or Publication Title: Chest
Publisher: American College of Chest Physicians
Refereed: Yes
ISSN: 0012-3692
Depositing User: Users 29196 not found.
Date Deposited: 22 Dec 2010 16:37
Last Modified: 31 Jul 2014 15:38
URI: http://usir.salford.ac.uk/id/eprint/12672

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