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Increased airway T regulatory cells in asthmatic subjects

Smyth, LJC, Eustace, A, Kolsum, U, Blaikely, J and Singh, D 2010, 'Increased airway T regulatory cells in asthmatic subjects' , Chest, 138 (4) , pp. 905-912.

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Abstract

Background: T regulatory cells (Tregs) may play a role in the suppression of effector lymphocyte activity in asthma. We hypothesized that Treg numbers would be increased in patients with more severe asthma. We also investigated the regulatory function of CD4 cells by expression of cytotoxic T-lymphocyte antigen 4 (CTLA4), and the number of these cells that are intraepithelial lymphocytes expressing CD103. Objectives: The primary aim was to investigate Treg numbers in the BAL of patients with moderate to severe asthma compared with mild asthma and healthy controls. The secondary aim was to investigate BAL CD4+CTLA4 and CD4+CD103 expression in these groups. Methods: Airway lymphocytes obtained by bronchoscopy from healthy control subjects (six) and patients with mild (15) and moderate to severe (13) asthma were characterized by multiparameter flow cytometric analysis using three methods to determine the numbers of CD4+ Treg cells: CD4+CD25bright, CD4+CD25+CD127−, and CD4+FoxP3+. Results: The %CD4+FoxP3+ Tregs were increased in the BAL of patients with moderate to severe asthma (median 4.8%) compared with both mild asthma patients (median 2.5%, P = .03) and healthy subjects (median 0.95, P = .003). Similar findings were observed for CD4+CD25+CD127− Treg numbers, but not CD4CD25bright. CD4+ CTLA4 and CD103 expressions were raised in moderate to severe asthma patients compared with those with mild asthma and healthy controls. Conclusions: The number of cells displaying regulatory capacity, either through FoxP3 expression or CTLA4 expression, is increased in moderate to severe asthma. CD4+CD103+ intraepithelial lymphocytes can be retained at tissue sites of inflammation; our findings indicate a role for these cells in asthma pathophysiology.

Item Type: Article
Themes: Subjects / Themes > Q Science > QR Microbiology
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Journal or Publication Title: Chest
Publisher: American College of Chest Physicians
Refereed: Yes
ISSN: 0012-3692
Depositing User: Users 29196 not found.
Date Deposited: 22 Dec 2010 16:58
Last Modified: 20 Aug 2013 17:44
URI: http://usir.salford.ac.uk/id/eprint/12674

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