Skip to the content

An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site

Brooks, DR, Appleford, PJ, Murray, L and Isaac, RE 2003, 'An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site' , The Journal of Biological Chemistry, 278 (52) , pp. 52340-52346.

Full text not available from this repository. (Request a copy)

Abstract

Genome sequence analyses predict many proteins that are structurally related to proteases but lack catalytic residues, thus making functional assignment difficult. We show that one of these proteins (ACN-1), a unique multi-domain angiotensin-converting enzyme (ACE)-like protein from Caenorhabditis elegans, is essential for larval development and adult morphogenesis. Green fluorescent protein-tagged ACN-1 is expressed in hypodermal cells, the developing vulva, and the ray papillae of the male tail. The hypodermal expression of acn-1 appears to be controlled by nhr-23 and nhr-25, two nuclear hormone receptors known to regulate molting in C. elegans. acn-1(RNAi) causes arrest of larval development because of a molting defect, a protruding vulva in adult hermaphrodites, severely disrupted alae, and an incomplete seam syncytium. Adult males also have multiple tail defects. The failure of the larval seam cells to undergo normal cell fusion is the likely reason for the severe disruption of the adult alae. We propose that alteration of the ancestral ACE during evolution, by loss of the metallopeptidase active site and the addition of new protein modules, has provided opportunities for novel molecular interactions important for post-embryonic development in nematodes.

Item Type: Article
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: The Journal of Biological Chemistry
Publisher: American Society for Biochemistry and Molecular Biology
Refereed: Yes
ISSN: 1083351X
Depositing User: H Kenna
Date Deposited: 07 Aug 2007 11:25
Last Modified: 20 Aug 2013 16:45
URI: http://usir.salford.ac.uk/id/eprint/129

Actions (login required)

Edit record (repository staff only)