Shakeri, J and Foster, HA 2007, 'Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects' , Enzyme and Microbial Technology, 40 (4) , pp. 961-968.
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Two strains of Trichoderma harzianum, 101645, an insect pathogen and 206040, used for biological control of fungal plant pathogens were investigated for the production of serine protease, chitinase and antibiotic activity in relation to entomopathogenicity. Both strains produced serine protease with a Mr of 31 kDa and chitinase with a Mr of 44 kDa. Enzymes from both strains had similar characteristics and were produced during the growth phase. Both strains also produced peptaibols active against fungi in late growth and stationary phases which differed in their amino-alcohol content. The peptaibols were insecticidal when fed to larvae of Tenebrio molitor or when applied to the cuticle together with the serine protease. The results suggest that the virulence factors involved in biocontrol are the same as those for insect pathogenicity. This may affect the use of Trichoderma spp. for biocontrol as there may be effects on non-target insect species. Â© 2006 Elsevier Inc. All rights reserved.
|Additional Information:||Cited By (since 1996): 10 Export Date: 2 March 2011 Source: Scopus CODEN: EMTED Language of Original Document: English Correspondence Address: Foster, H.A.; Biomedical Sciences Research Institute, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester M5 4WT, United Kingdom; email: firstname.lastname@example.org Chemicals/CAS: chitinase, 9001-06-3; serine proteinase, 37259-58-8|
|Uncontrolled Keywords:||Biological control Chitinase Entomopathogen Peptaibol Protease Trichoderma harzianum Alcohols Antibiotics Biocontrol Enzymes Insect control Pathology Entomopathogens Insect pathogens Peptaibols active Serine proteases Fungi aminoalcohol antibiotic agent serine proteinase virulence factor antimicrobial activity article biological pest control controlled study cuticle entomopathogeny fungal strain fungus growth larva nonhuman pathogenicity protein degradation Tenebrio tenebrio molitor Hexapoda Hypocrea lixii Trichoderma|
|Themes:||Health and Wellbeing|
|Schools:||Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre|
|Journal or Publication Title:||Enzyme and Microbial Technology|
|Depositing User:||Professor Howard Anthony Foster|
|Date Deposited:||11 Oct 2011 11:05|
|Last Modified:||21 Sep 2015 10:31|
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