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Measuring whole-body neutrophil redistribution using a dedicated whole-body counter and ultra-low doses of 111 Indium

Szczepura, K, Ruparelia, P, Solanki, CK, Balan, K, Newbold, P, Summers, C, Chilvers, ER and Peters, AM 2011, 'Measuring whole-body neutrophil redistribution using a dedicated whole-body counter and ultra-low doses of 111 Indium' , European Journal of Clinical Investigation, 41 (1) , pp. 77-83.

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    Abstract

    Background - There is increasing interest in the ‘homing’ of neutrophils to bone marrow. The aim of this study was to measure the whole-body redistribution of 111In using a whole-body counter following the administration of ultra-small activities of 111 In-labelled neutrophils. Methods The detectors of a dedicated whole-body counter were fitted with lead collimators. Whole-body 111 In distribution was recorded at 45 min, 24 h, and 2, 4, 7 and 10 days after administration of 111 In-labelled neutrophils (0.29–0.74 MBq) in eight healthy non-smokers, five healthy smokers, eight patients with inactive bronchiectasis, three with asthma and nine with chronic obstructive pulmonary disease (COPD). Results - Intravascular 45-min 111In-labelled neutrophil recovery was not significantly different between groups, ranging from 33 (SD 8%) in healthy smokers to 45 (14%) in healthy non-smokers (P > 0.05). Peaks were identified on the whole body count profile corresponding to the chest, upper abdomen (liver ⁄ spleen) and pelvis (bone marrow). 111 In distribution changed between 45 min and 24 h and then remained stable thereafter. Peak chest counts increased ~ 1.5-fold between 45 min and 24 h, whereas upper abdominal peak counts decreased by ~ 25% with no significant inter-group differences. The increment in pelvic counts (~2.7-fold) was similar between groups, except COPD patients, in whom it was 2.04 (0.35; P < 0.02 vs. healthy participants). Conclusions - Assuming neutrophils are distributed only between blood, liver, spleen and bone marrow, the data suggest that marrow pools 25% and destroys 67% of circulating neutrophils, rising in COPD to 40% and 80%, respectively, possibly as a result of the effects on marrow of chronic hypoxaemia.

    Item Type: Article
    Uncontrolled Keywords: Bone marrow, 111 In-neutrophils, liver, spleen, whole-body counting
    Themes: Health and Wellbeing
    Schools: Colleges and Schools > College of Health & Social Care
    Colleges and Schools > College of Health & Social Care > School of Health Sciences
    Journal or Publication Title: European Journal of Clinical Investigation
    Publisher: Wiley-Blackwell
    Refereed: Yes
    ISSN: 0014-2972
    Depositing User: RH Shuttleworth
    Date Deposited: 16 May 2011 11:05
    Last Modified: 20 Aug 2013 17:52
    URI: http://usir.salford.ac.uk/id/eprint/15789

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