Skip to the content

Quantification of neutrophil migration into the lungs of patients with chronic obstructive pulmonary disease

Ruparelia, P, Szczepura, K, Summers, C, Solanki, C, Balan, K, Newbold, P, Bilton, D, Peters, A and Chilvers, E 2011, 'Quantification of neutrophil migration into the lungs of patients with chronic obstructive pulmonary disease' , European Journal of Nuclear Medicine and Molecular Imaging, 38 (5) , pp. 911-919.

Full text not available from this repository. (Request a copy)

Abstract

Objective: to quantify neutrophil migration into the lungs of patients with chronic pulmonary obstructive disease (COPD). Methods: neutrophil loss via airways was assessed by dedicated whole-body counting 45 min, 24 h and 2, 4, 7 and 10 days after injection of very small activities of 111In-labelled neutrophils in 12 healthy nonsmokers, 5 healthy smokers, 16 patients with COPD (of whom 7 were ex-smokers) and 10 patients with bronchiectasis. Lung accumulation of 99mTc-labelled neutrophils was assessed by sequential SPECT and Patlak analysis in six COPD patients and three healthy nonsmoking subjects. Results: whole body 111In counts, expressed as percentages of 24 h counts, decreased in all subjects. Losses at 7 days (mean ± SD) were similar in healthy nonsmoking subjects (5.5 ± 1.5%), smoking subjects (6.5 ± 4.4%) and ex-smoking COPD patients (5.8 ± 1.5%). In contrast, currently smoking COPD patients showed higher losses (8.0 ± 3.0%) than healthy nonsmokers (p = 0.03). Two bronchiectatic patients lost 25% and 26%, indicating active disease; mean loss in the remaining eight was 6.9 ± 2.5%. The rate of accumulation of 99mTc-neutrophils in the lungs, determined by sequential SPECT, was increased in COPD patients (0.030–0.073 min−1) compared with healthy nonsmokers (0–0.002 min−1; p = 0.02). Conclusion: in patients with COPD, sequential SPECT showed increased lung accumulation of 99mTc-labelled neutrophils, while whole-body counting demonstrated subsequent higher losses of 111In-labelled neutrophils in patients who continued to smoke. Sequential SPECT as a means of quantifying neutrophil migration deserves further evaluation.

Item Type: Article
Uncontrolled Keywords: Neutrophils, granulocytes, COPD, whole-body counting, 111 In-tropolonate, 99mTc HMPAO
Themes: Health and Wellbeing
Schools: Colleges and Schools > College of Health & Social Care
Colleges and Schools > College of Health & Social Care > School of Health Sciences
Journal or Publication Title: European Journal of Nuclear Medicine and Molecular Imaging
Publisher: Springer Verlag
Refereed: Yes
ISSN: 1619-7070
Depositing User: RH Shuttleworth
Date Deposited: 08 Jun 2011 09:55
Last Modified: 20 Aug 2013 17:53
URI: http://usir.salford.ac.uk/id/eprint/15954

Actions (login required)

Edit record (repository staff only)