Ruparelia, P, Szczepura, K, Summers, C, Solanki, C, Balan, K, Newbold, P, Bilton, D, Peters, A and Chilvers, E 2011, 'Quantification of neutrophil migration into the lungs of patients with chronic obstructive pulmonary disease' , European Journal of Nuclear Medicine and Molecular Imaging, 38 (5) , pp. 911-919.

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Abstract

Objective: to quantify neutrophil migration into the lungs of patients with chronic pulmonary obstructive disease (COPD). Methods: neutrophil loss via airways was assessed by dedicated whole-body counting 45 min, 24 h and 2, 4, 7 and 10 days after injection of very small activities of 111In-labelled neutrophils in 12 healthy nonsmokers, 5 healthy smokers, 16 patients with COPD (of whom 7 were ex-smokers) and 10 patients with bronchiectasis. Lung accumulation of 99mTc-labelled neutrophils was assessed by sequential SPECT and Patlak analysis in six COPD patients and three healthy nonsmoking subjects. Results: whole body 111In counts, expressed as percentages of 24 h counts, decreased in all subjects. Losses at 7 days (mean ± SD) were similar in healthy nonsmoking subjects (5.5 ± 1.5%), smoking subjects (6.5 ± 4.4%) and ex-smoking COPD patients (5.8 ± 1.5%). In contrast, currently smoking COPD patients showed higher losses (8.0 ± 3.0%) than healthy nonsmokers (p = 0.03). Two bronchiectatic patients lost 25% and 26%, indicating active disease; mean loss in the remaining eight was 6.9 ± 2.5%. The rate of accumulation of 99mTc-neutrophils in the lungs, determined by sequential SPECT, was increased in COPD patients (0.030–0.073 min−1) compared with healthy nonsmokers (0–0.002 min−1; p = 0.02). Conclusion: in patients with COPD, sequential SPECT showed increased lung accumulation of 99mTc-labelled neutrophils, while whole-body counting demonstrated subsequent higher losses of 111In-labelled neutrophils in patients who continued to smoke. Sequential SPECT as a means of quantifying neutrophil migration deserves further evaluation.

Item Type:	Article
Uncontrolled Keywords:	Neutrophils, granulocytes, COPD, whole-body counting, 111 In-tropolonate, 99mTc HMPAO
Themes:	Health and Wellbeing
Schools:	Colleges and Schools > College of Health & Social Care Colleges and Schools > College of Health & Social Care > School of Health Sciences
Journal or Publication Title:	European Journal of Nuclear Medicine and Molecular Imaging
Publisher:	Springer Verlag
Refereed:	Yes
ISSN:	1619-7070
Depositing User:	RH Shuttleworth
Date Deposited:	08 Jun 2011 09:55
Last Modified:	08 Jun 2011 09:55
URI:	http://usir.salford.ac.uk/id/eprint/15954

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