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Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging

Mitchell, A, Wall, JE, Murray, JG and Morgan, CG 2002, 'Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging' , Journal of Microscopy, 206 (3) , pp. 225-232.

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Abstract

In this paper a novel approach to frequency-domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the sensitivity of the CCD at radio frequency. The modulation enhances the noise performance of the CCD, in contrast to the deterioration in performance seen when an intensifier stage is similarly modulated. The new technology has potential applications to a wide range of assays as well as in conventional FLIM applications. Unlike intensifier-based systems, the directly modulated CCD is physically small, inexpensive, robust and offers superior resolution and noise performance.

Item Type: Article
Uncontrolled Keywords: CCD, FLIM, fluorescence lifetime imaging, modulation, phase-shift, time-resolved detection
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects / Themes > Q Science > QC Physics
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Journal of Microscopy
Publisher: Blackwell Publishing
Refereed: Yes
ISSN: 00222720
Depositing User: H Kenna
Date Deposited: 08 Aug 2007 10:14
Last Modified: 20 Aug 2013 16:45
URI: http://usir.salford.ac.uk/id/eprint/160

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