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Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera

Mitchell, A, Wall, JE, Murray, JG and Morgan, CG 2002, 'Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera' , Journal of Microscopy, 206 (3) , pp. 233-238.

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Abstract

CCD cameras coupled optically to gated image intensifiers have been used for fast time-resolved measurements for some years. Image intensifiers have disadvantages, however, and for some applications it would be better if the image sensor could be gated directly at high speed. Control of the 'charge drain' function on an interline-transfer CCD allows the sensor to be switched rapidly from an insensitive state. The temporal and spatial properties of the charge drain are explored in the present paper and it is shown that nanosecond time resolution with acceptable spatial uniformity can be achieved for a small commercial sensor. A fluorescence lifetime imaging system is demonstrated, based on a repetitively pulsed laser excitation source synchronized to the CCD control circuitry via a programmable delay unit.

Item Type: Article
Uncontrolled Keywords: CCD, FLIM, fluorescence lifetime imaging, gating, time-resolved detection
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Journal of Microscopy
Publisher: Blackwell Publishing
Refereed: Yes
ISSN: 00222720
Depositing User: H Kenna
Date Deposited: 08 Aug 2007 10:17
Last Modified: 20 Aug 2013 16:45
URI: http://usir.salford.ac.uk/id/eprint/161

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