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Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging

Bisby, RH, Botchway, SW, Crisostomo, AG, Karolin, J, Parker, AW and Schroder, L 2010, 'Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging' , Spectroscopy, 24 (1-2) , pp. 137-142.

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        Abstract

        Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the fluorescence spectrum and an increase in fluorescence lifetime. Quenching of propranolol fluorescence by iodide is used to demonstrate interaction with beta cyclodextrin. At high concentrations, self –quenching of propranolol fluorescence was also observed with kq 2.5x109 dm3 mol-1 s-1. Two-photon excited (630 nm) fluorescence lifetime imaging of propranolol in cells showed propranolol to be widely distributed in the cell cytoplasm, with fluorescence lifetimes shorter than in solution. The results suggest that intracellular propranolol is mainly confined within the aqueous cytoplasm and rather than membrane associated.

        Item Type: Article
        Uncontrolled Keywords: Propranolol, fluorescence, lifetime, quenching, micelle, cyclodextrin, imaging, live cell
        Themes: Health and Wellbeing
        Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
        Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
        Journal or Publication Title: Spectroscopy
        Publisher: IOS Press
        Refereed: Yes
        ISSN: 0712-4813
        Related URLs:
        Depositing User: RH Bisby
        Date Deposited: 28 Sep 2011 12:49
        Last Modified: 20 Aug 2013 18:10
        URI: http://usir.salford.ac.uk/id/eprint/17769

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