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Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus

Taherkhani, H, Zeyhle, E and Rogan, MT 2007, 'Antibody responses in human cystic hydatid disease to the laminated layer of echinococcus granulosus' , Parasitology Research, 101 (3) , pp. 647-652. (In Press)

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Abstract

The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and were found to contain bands at 55 and 25-29 kDa, which reacted with an anti-sheep IgG antibody probe, indicating that these were likely to be host-contaminating components within the layer. However, the same bands were also recognised by a significant proportion of human hydatid patients, particularly by IgG4 antibodies, and not by negative control individuals. These individuals did not recognise immunoglobulin heavy and light chains in a sheep serum extract in the same manner. It seems likely that there are either host or parasite antigenic components at similar molecular weights or that certain parasite antigens may share epitopes with sheep immunoglobulins. The antigens at 25-29 kDa were found to be glycoproteins by lectin blot analysis and may be important markers of disease status.

Item Type: Article
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Parasitology Research
Publisher: Springer Berlin / Heidelberg
Refereed: Yes
ISSN: 09320113
Depositing User: H Kenna
Date Deposited: 08 Aug 2007 12:48
Last Modified: 20 Aug 2013 16:45
URI: http://usir.salford.ac.uk/id/eprint/178

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