Schistosoma mansoni: egg-induced downregulation of hepatic stellate cell activation and fibrogenesis
Anthony, BJ, Mathieson, W, de Castro-Borges, W and Allen, JT 2010, 'Schistosoma mansoni: egg-induced downregulation of hepatic stellate cell activation and fibrogenesis' , Experimental parasitology, 124 (4) , pp. 409-420.
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Eggs of Schistosoma mansoni trapped in human liver can lead to fibrosis. Since liver fibrosis requires activation of hepatic stellate cells (HSC) from a quiescent to a myofibroblastic phenotype, we investigated the effects of S. mansoni eggs on this process using in vitro co-cultures with human HSC and evaluated established biomarkers for activation and fibrosis. HSC demonstrate significantly reduced expression of alpha-smooth muscle actin (p<0.001), connective tissue growth factor (p<0.01) and type I collagen (p<0.001) but significantly increased expression of peroxisome proliferator-activated receptor-gamma (p<0.01). Morphologically, HSC exhibited elongated fine cellular processes and reduced size, increased accumulation of lipid droplets and reduced expression and organization of alpha-smooth muscle actin and F-actin stress fibres. Additionally, schistosome eggs prevented the HSC fibrogenic response to exogenous transforming growth factor-beta. In summary, schistosome eggs blocked fibrogenesis in HSC, a finding which may have implications for our understanding of the fibrotic pathology in S. mansoni infections.
|Themes:||Health and Wellbeing|
|Schools:||Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre|
|Journal or Publication Title:||Experimental parasitology|
|Depositing User:||JT Allen|
|Date Deposited:||06 Oct 2011 09:20|
|Last Modified:||23 May 2014 12:55|
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