Skip to the content

Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion

Hogervorst, PAM, Ferry, N, Gatehouse, AMR, Wäckers, FL and Romeis, J 2006, 'Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion' , Journal of insect physiology, 52 (6) , pp. 614-24.

[img] PDF - Published Version
Restricted to Repository staff only

Download (367kB) | Request a copy

    Abstract

    Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.

    Item Type: Article
    Themes: Built and Human Environment
    Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
    Journal or Publication Title: Journal of insect physiology
    Publisher: Elsevier
    Refereed: Yes
    ISSN: 0022-1910
    Depositing User: Dr Natalie Ferry
    Date Deposited: 06 Oct 2011 15:20
    Last Modified: 20 Aug 2013 18:11
    URI: http://usir.salford.ac.uk/id/eprint/17964

    Actions (login required)

    Edit record (repository staff only)

    Downloads per month over past year

    View more statistics