Elkord, E and Burt, D 2011, 'Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells ' , Scandinavian Journal Of Immunology, 74 (4) , pp. 419-422.

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Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Item Type:	Article
Themes:	Subjects outside of the University Themes
Schools:	Colleges and Schools > College of Science & Technology Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	Scandinavian Journal Of Immunology
Publisher:	Blackwell Publishing
Refereed:	Yes
ISSN:	0300-9475
Depositing User:	E Elkord
Date Deposited:	11 Oct 2011 16:18
Last Modified:	11 Oct 2011 16:18
URI:	http://usir.salford.ac.uk/id/eprint/18228

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