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Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

Elkord, E and Burt, D 2011, 'Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells ' , Scandinavian Journal Of Immunology, 74 (4) , pp. 419-422.

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Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Item Type: Article
Themes: Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Scandinavian Journal Of Immunology
Publisher: Blackwell Publishing
Refereed: Yes
ISSN: 0300-9475
Depositing User: E Elkord
Date Deposited: 11 Oct 2011 16:18
Last Modified: 20 Aug 2013 18:13
URI: http://usir.salford.ac.uk/id/eprint/18228

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