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Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Elkord, E 2009, 'Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation ' , Journal of Immunological Methods, 347 (1-2) , pp. 87-90.

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    Abstract

    Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

    Item Type: Article
    Themes: Health and Wellbeing
    Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
    Journal or Publication Title: Journal of Immunological Methods
    Publisher: Elsevier
    Refereed: Yes
    ISSN: 0022-1759
    Depositing User: E Elkord
    Date Deposited: 12 Oct 2011 11:55
    Last Modified: 20 Aug 2013 18:13
    URI: http://usir.salford.ac.uk/id/eprint/18237

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