Skip to the content

Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer

Elkord, E, Rowbottom, A, Kynaston, H and Wiiliams, P 2006, 'Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer' , Clinical Immunology, 120 (1) , pp. 91-98.

[img] PDF - Published Version
Restricted to Repository staff only

Download (260kB) | Request a copy

    Abstract

    Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level.

    Item Type: Article
    Themes: Health and Wellbeing
    Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
    Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
    Journal or Publication Title: Clinical Immunology
    Publisher: Elsevier
    Refereed: Yes
    ISSN: 1521-6616
    Depositing User: E Elkord
    Date Deposited: 13 Oct 2011 11:02
    Last Modified: 20 Aug 2013 18:13
    URI: http://usir.salford.ac.uk/id/eprint/18289

    Actions (login required)

    Edit record (repository staff only)

    Downloads per month over past year

    View more statistics