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Development of a species-specific coproantigen ELISA for human taenia solium taeniasis

Guezala, MC, Rodriguez, S, Zamora, H, Garcia, HH, Gonzalez, AE, Tembo, A, Allan, JC and Craig, PS 2009, 'Development of a species-specific coproantigen ELISA for human taenia solium taeniasis' , The American Journal of Tropical Medicine & Hygiene, 81 (3) , pp. 433-437.

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    Abstract

    Taenia solium causes human neurocysticercosis and is endemic in underdeveloped countries where backyard pig keeping is common. Microscopic fecal diagnostic methods for human T. solium taeniasis are not very sensitive, and Taenia saginata and Taenia solium eggs are indistinguishable under the light microscope. Coproantigen (CoAg) ELISA methods are very sensitive, but currently only genus (Taenia) specific. This paper describes the development of a highly species-specific coproantigen ELISA test to detect T. solium intestinal taeniasis. Sensitivity was maintained using a capture antibody of rabbit IgG against T. solium adult whole worm somatic extract, whereas species specificity was achieved by utilization of an enzyme-conjugated rabbit IgG against T. solium adult excretory-secretory (ES) antigen. A known panel of positive and negative human fecal samples was tested with this hybrid sandwich ELISA. The ELISA test gave 100% specificity and 96.4% sensitivity for T. solium tapeworm carriers (N = 28), with a J index of 0.96. This simple ELISA incorporating anti-adult somatic and anti-adult ES antibodies provides the first potentially species-specific coproantigen test for human T. solium taeniasis.

    Item Type: Article
    Themes: Subjects outside of the University Themes
    Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
    Journal or Publication Title: The American Journal of Tropical Medicine & Hygiene
    Publisher: American Society of Tropical Medicine & Hygiene
    Refereed: Yes
    ISSN: 0002-9637
    Depositing User: Users 29196 not found.
    Date Deposited: 20 Dec 2011 14:00
    Last Modified: 03 Jul 2014 10:57
    URI: http://usir.salford.ac.uk/id/eprint/19240

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