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Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges

Nirmalan, NJ, Harnden, P, Selby, PJ and Banks, RE 2008, 'Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges' , Molecular BioSystems, 4 (7) , pp. 712-720.

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    Abstract

    The significant potential of tissue-based proteomic biomarker studies can be restricted by difficulties in accessing samples in optimal fresh-frozen form. While archival formalinfixed tissue collections with attached clinical and outcome data represent a valuable alternate resource, the use of formalin as a fixative which induces protein cross-linking, has generally been assumed to render them unsuitable for proteomic studies. However, this view has been challenged recently with the publication of several papers accomplishing variable degrees of heat-induced reversal of cross-links. Although still in its infancy and requiring the quantitative optimisation of several critical parameters, formalin-fixed tissue proteomics holds promise as a powerful tool for biomarker-driven translational research. Here, we critically review the current status of research in the field, highlighting challenges which need to be addressed for robust quantitative application of protocols to ensure confident high impact inferences can be made.

    Item Type: Article
    Themes: Subjects outside of the University Themes
    Schools: Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
    Journal or Publication Title: Molecular BioSystems
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    ISSN: 1742-206X
    Depositing User: Users 29196 not found.
    Date Deposited: 20 Dec 2011 15:57
    Last Modified: 20 Aug 2013 18:19
    URI: http://usir.salford.ac.uk/id/eprint/19246

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