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Screening for Echinococcus granulosus in dogs: comparison between arecoline purgation, coproELISA and coproPCR with necropsy in pre-patent infection

Lahmar, S, Lahmar, S, Boufana, B, Bradshaw, H and Craig, PS 2007, 'Screening for Echinococcus granulosus in dogs: comparison between arecoline purgation, coproELISA and coproPCR with necropsy in pre-patent infection' , Veterinary Parasitology, 144 (3-4) , pp. 287-292.

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Abstract

Echinococcus granulosus is an important zoonotic infection of dogs. The purpose of the present study assessed the performance of two laboratory diagnostic methods with arecoline purgation and necropsy in infected dogs. In total 65 dogs were successfully experimentally infected with protoscoleces of E. granulosus from ovine infection. At 14–34 days post-infection groups of dogs were purged with arecoline hydrobromide and then necropsied. Faecal samples were tested at weekly intervals by coproantigen ELISA and coproPCR. The necropsy infection rate with E. granulosus was 89.2%. Only 43% of dogs were successfully purged after one arecoline dose; this percentage increased to 76.9% for two doses of arecoline purgation. E. granulosus coproantigen was detected by coproELISA in 82.8% of faeces. The positive and negative predictive values for coproantigen ELISA were 96 and 44.4% respectively. E. granulosus DNA was detected in pre-patent faecal samples by coproPCR in 25.9% of dogs. These results indicate that coproELISA is more sensitive than arecoline purgation for the detection of pre-patent E. granulosus infection in dogs. CoproPCR detected E. granulosus DNA in dog faeces by 21 days post-infection before egg production.

Item Type: Article
Uncontrolled Keywords: Echinococcosis, dogs, pre-patent infection, arecoline, coproELISA, coproPCR, necropsy
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects / Themes > R Medicine > R Medicine (General)
Health and Wellbeing
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title: Veterinary Parasitology
Publisher: Elsevier
Refereed: Yes
ISSN: 03044017
Depositing User: H Kenna
Date Deposited: 08 Aug 2007 15:43
Last Modified: 20 Aug 2013 16:45
URI: http://usir.salford.ac.uk/id/eprint/195

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