Wang, Y, Bradshaw, H, Rogan, MT and Craig, PS 2002, 'Rapid dot-ELISA for the detection of specific antigens in the cyst fluid from human cases of cystic echinococcosis.' , Annals of Tropical Medicine and Parasitology, 96 (7) , pp. 691-694.

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Abstract

A rapid dot-ELISA was developed for the detection of specific antigens in samples of cyst fluid from human cases of Echinococcus granulosus infection, permitting the confirmation of cystic echinococcosis (CE). Peroxidase-conjugated antibodies against antigen B (derived from the fluid in the cysts of sheep infected with E. granulosus) were used to test cyst-fluid samples from 22, surgically confirmed cases of human CE and 21 domestic animals (horses, sheep, buffalo, a cow and a camel) with CE. All of these samples were found to be strongly positive in the dot-ELISA, by direct reading with the naked eye. In contrast, fluid samples from seven, non-parasitic liver cysts of human origin were all negative, and a sample taken from the residual cavity left in a patient after previous CE surgery showed a weakly positive reaction. The antigen-detection assay, which can be completed within 10min, may be a useful 'bedside' test for the differential diagnosis of cysts during surgery or percutaneous treatment for suspected CE.

Item Type:	Article
Themes:	Subjects / Themes > R Medicine > R Medicine (General) Health and Wellbeing
Schools:	Colleges and Schools > College of Science & Technology Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	Annals of Tropical Medicine and Parasitology
Publisher:	Elsevier
Refereed:	Yes
ISSN:	1364-8594
Depositing User:	H Kenna
Date Deposited:	10 Aug 2007 14:09
Last Modified:	08 Mar 2012 10:06
URI:	http://usir.salford.ac.uk/id/eprint/243

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