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Monitoring the direct and indirect damage of DNA bases and polynucleotides by using time-resolved infrared spectroscopy

Kuimova, MK, Cowan, AJ, Matousek, P, PARKER, AW, Sun, XZ, Towrie, M and George, MW 2006, 'Monitoring the direct and indirect damage of DNA bases and polynucleotides by using time-resolved infrared spectroscopy' , Proceedings of the National Academy of Sciences of the United States of America (PNAS) ISSN 1091-6490, 103 (7) , pp. 2150-2153.

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Abstract

The nucleotide 5'-dGMP and polynucleotide poly(dGdC)·poly(dGdC) have been irradiated by using a 200-fs, 200-nm laser pulses and spectrally characterized by using time-resolved infrared spectroscopy. Under the experimental conditions, 200-nm excitation generates both electronic excited states and radical cations through photoionization; the former decay rapidly to vibrationally hot ground state. By using infrared signatures we have been able to follow these processes, and at time scales of >1 ns we observe an infrared marker band at 1,702 cm–1 within both 5'-dGMP and the polynucleotide assigned to a photoionized product of guanine. This transient has also been reproduced through indirect chemistry through the reaction with photogenerated carbonate radical with 5'-dGMP. The ability to use time-resolved infrared spectroscopy in this way paves the way for developing solution-phase studies to investigate both direct and indirect radiation chemistry of DNA. DNA damage | electron transfer | guanine radical cation

Item Type: Article
Themes: Subjects / Themes > Q Science > Q Science (General)
Subjects outside of the University Themes
Schools: Colleges and Schools > College of Science & Technology
Colleges and Schools > College of Science & Technology > School of Environment and Life Sciences
Journal or Publication Title: Proceedings of the National Academy of Sciences of the United States of America (PNAS) ISSN 1091-6490
Publisher: National Academy of Sciences
Refereed: Yes
ISSN: 1091-6490
Depositing User: H Kenna
Date Deposited: 10 Aug 2007 14:55
Last Modified: 20 Aug 2013 16:46
URI: http://usir.salford.ac.uk/id/eprint/247

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