Increased susceptibility to Trichuris muris infection and exacerbation of colitis in Mdr1a-/- mice

Bhardwaj, EK, Else, KJ, Rogan, T and Warhurst, G 2014, 'Increased susceptibility to Trichuris muris infection and exacerbation of colitis in Mdr1a-/- mice' , World Journal of Gastroenterology, 20 (7) , p. 1797.

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AIM: To investigate the influence of Trichuris muris (T. muris) infection in a mouse model of genetic susceptibility to inflammatory bowel disease, Mdr1a-/-. METHODS: Mdr1a-/- mice were housed under specific pathogen free conditions to slow the development of colitis and compared to congenic FVB controls. Mice were infected with approximately 200 embryonated ova from T. muris and assessed for worm burden and histological and functional markers of gut inflammation on day 19 post infection. RESULTS: Mdr1a-/- mice exhibited a marked increase in susceptibility to T. muris infection with a 10-fold increase in colonic worm count by day 19 pi compared to FVB controls. Prior to infection, Mdr1a-/- exhibited low-level mucosal inflammation with evidence of an enhanced Th1 environment. T. muris infection accelerated the progression of colitis in Mdr1a-/- as evidenced by marked increases in several indicators including histological damage score, mucosal CD4+ T-cell and DC infiltration and dramatically increased production of pro-inflammatory cytokines. CONCLUSION: These data provide further evidence of the complex interaction between T. muris and an inflammatory bowel disease (IBD)-susceptible host which may have relevance to the application of helminth therapy in the treatment of human IBD.

Item Type: Article
Uncontrolled Keywords: Helminth, Colitis, Inflammatory bowel disease, P-glycoprotein, Mdr1a
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Schools > School of Environment and Life Sciences
Journal or Publication Title: World Journal of Gastroenterology
Publisher: Baishideng Publishing Group Co. Limited
Refereed: Yes
ISSN: 1007-9327
Depositing User: S Rafiq
Date Deposited: 15 Apr 2014 17:31
Last Modified: 17 Oct 2016 14:12

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