Skip to the content

Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis

Xu, Lina, Wang, Z, Zhang, J, Ferry, N, Edwards, MG, Gatehouse, AMR and He, K 2014, 'Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis' , Journal of Invertebrate Pathology, 115 , pp. 95-98.

Full text not available from this repository. (Request a copy)

Abstract

The full-length cDNA of four Ofapn aminopeptidases were cloned and sequenced from susceptible and resistant Ostrinia furnacalis strains. Four sequences were identified as APN because they shared the common structural features with APN from Lepidoptera, including the signal peptide, GPI anchor signal, the zinc binding/gluzincin motif HEX2HX18E and the gluzincin aminopeptidase motif GAMEN. Compared with APN sequences from the susceptible strain, there were 9, 5, 10 and 12 amino acid variations in the deduced protein sequences from the resistant strain. There were also differences in mRNA expression of the four Ofapn genes between resistant and susceptible O. furnacalis strains.

Item Type: Article
Uncontrolled Keywords: Aminopeptidase N; cDNA; mRNA expression; Bt receptors; Ostrinia furnacalis
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Journal of Invertebrate Pathology
Publisher: Elsevier
Refereed: Yes
ISSN: 0022-2011
Depositing User: S Rafiq
Date Deposited: 07 May 2014 09:59
Last Modified: 30 Nov 2015 23:54
URI: http://usir.salford.ac.uk/id/eprint/31773

Actions (login required)

Edit record (repository staff only) Edit record (repository staff only)