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Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis

Feng, X, Wen, H, Zhang, Z, Chen, X, Ma, X, Zhang, J, Qi, X, Bradshaw, H, Vuitton, D and Craig, PS 2010, 'Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis' , Acta Tropica, 113 (2) , pp. 114-120.

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Abstract

A new 3-min rapid dot immunogold filtration assay (DIGFA) for serodiagnosis of human cystic and alveolar echinococcosis was developed using four native antigen preparations: crude and partially purified hydatid cyst fluid extracts from Echinococcus granulosus (EgCF and AgB), E. granulosus protoscolex extract (EgP) and Echinococcus multilocularis metacestode antigen (Em2). The overall sensitivity of DIGFA in a hospital diagnostic setting was 80.7% for human cystic echinococcosis (CE) (n=857) and 92.9% for human alveolar echinococcosis (AE) (n=42). Highest specificity was 93.4% with AgB extract for CE, and 90.3% with Em2 antigen for AE when CE versus AE cross-reactivity was excluded. Anti-AgB antibodies were present in 35.5% of AE cases and anti-Em2 in 7.4% of CE cases. In endemic communities in northwest China screened for echinococcosis, the sensitivity of DIGFA ranged from 71.8% to 90.7% in comparison to abdominal ultrasound; specificity for CE using AgB was 94.6% and for AE using Em2 was 97.1%. This simple eye-read rapid test can be used for both clinical diagnostic support, as well as in conjunction with ultrasound for mass screening in endemic CE and AE areas.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Acta Tropica
Publisher: Elsevier
Refereed: Yes
ISSN: 0001706X
Depositing User: Institutional Repository
Date Deposited: 11 Sep 2014 16:29
Last Modified: 05 Apr 2016 18:16
URI: http://usir.salford.ac.uk/id/eprint/32336

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