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Use of competition dialysis in the discovery of G-quadruplex selective ligands

Ragazzon, PA and Chaires, J 2007, 'Use of competition dialysis in the discovery of G-quadruplex selective ligands' , Methods, 43 (4) , pp. 313-323.

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G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand oreintations, to bimolecular dimeric structures, and finally to tetramolecular parallel-stranded structures. These diverse structures present numerous potential small molecule binding sites with distinctive properties. There is mounting evidence for important functional roles for G-quadruplex structures in biology. G-quadruplexes may participate in the maintenance of telomeres, in transcriptional regulation and, in mRNA, may act to modulate translation. G-quadruplexes thus represent an attractive target for new small-molecule therapeutic agents. Competition dialysis provides a useful tool for the discovery of small molecules that selectively recognize the unique structural features of G-quadruplexes. The principles and practice of the competition dialysis experiment are described here.

Item Type: Article
Themes: Health and Wellbeing
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Methods
Publisher: Elsevier
Refereed: Yes
ISSN: 1046-2023
Related URLs:
Funders: Brown Cancer Center
Depositing User: PA Ragazzon
Date Deposited: 26 Jan 2015 13:51
Last Modified: 26 Jan 2015 13:51

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