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Circular dichroism to determine binding mode and affinity of ligand-DNA interactions

Garbett, N, Ragazzon, PA and Chaires, J 2007, 'Circular dichroism to determine binding mode and affinity of ligand-DNA interactions' , Nature Protocols, 2 (12) , pp. 3166-3172.

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Abstract

Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand-DNA interactions and takes approximately 7.5 h.

Item Type: Article
Themes: Health and Wellbeing
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Nature Protocols
Publisher: Nature Publishing Group
Refereed: Yes
ISSN: 1754-2189
Related URLs:
Funders: Brown Cancer Center
Depositing User: PA Ragazzon
Date Deposited: 26 Jan 2015 13:54
Last Modified: 26 Jan 2015 13:54
URI: http://usir.salford.ac.uk/id/eprint/33309

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