Circular dichroism to determine binding mode and affinity of ligand-DNA interactions
Garbett, N, Ragazzon, PA and Chaires, J 2007, 'Circular dichroism to determine binding mode and affinity of ligand-DNA interactions' , Nature Protocols, 2 (12) , pp. 3166-3172.
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Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand-DNA interactions and takes approximately 7.5 h.
|Themes:||Health and Wellbeing|
|Schools:||Schools > School of Environment and Life Sciences > Biomedical Research Centre|
|Journal or Publication Title:||Nature Protocols|
|Publisher:||Nature Publishing Group|
|Funders:||Brown Cancer Center|
|Depositing User:||PA Ragazzon|
|Date Deposited:||26 Jan 2015 13:54|
|Last Modified:||26 Jan 2015 13:54|
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