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Ca analysis: an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis

Greensmith, DJ 2014, 'Ca analysis: an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis' , Computer methods and programs in biomedicine, 113 (1) , pp. 241-50.

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Abstract

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological information. The program performs two fundamental processes. (1) It can prepare the raw signal by several methods. (2) It can then be used to analyze the prepared data to provide information such as absolute intracellular Ca levels. Also, the rates of change of Ca can be measured using multiple, simultaneous regression analysis. I demonstrate that this program performs equally well as commercially available software, but has numerous advantages, namely creating a simplified, self-contained analysis workflow.

Item Type: Article
Themes: Health and Wellbeing
Subjects outside of the University Themes
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Computer methods and programs in biomedicine
Publisher: Elsevier
Refereed: Yes
ISSN: 1872-7565
Related URLs:
Funders: British Heart Foundation
Depositing User: D Greensmith
Date Deposited: 21 Jan 2015 12:02
Last Modified: 23 Jan 2015 10:04
URI: http://usir.salford.ac.uk/id/eprint/33360

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