Scherer, KM, Bisby, RH, Botchway, SW, Hadfield, JA, Haycock, JW and Parker, AW 2015, 'Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation' , Journal of Biomedical Optics, 20 (7) , p. 78003.
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The uptake of E-combretastatins, potential pro-drugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625 nm ultrafast femtosecond laser pulses. Uptake is initially observed at the spheroid periphery but extends to the spheroid core within 30 minutes. Using agarose gels as a three-dimensional model, the conversion of Z(trans) E(cis) via two-photon photoisomerization is demonstrated and the location of this photochemical process may be selected precisely within the micron scale in all three dimensions at depths up to almost 2 mm. We discuss these results for enhanced tissue penetration at longer near-infrared wavelengths for cancer therapy and up to three-photon excitation and imaging using 930 nm laser pulses with suitable combretastatin analogues.
|Schools:||Schools > School of Environment and Life Sciences > Biomedical Research Centre|
|Journal or Publication Title:||Journal of Biomedical Optics|
|Publisher:||Society of Photo-optical Instrumentation Engineers (SPIE)|
|Funders:||Science and Technology Facilities Council|
|Depositing User:||Institutional Repository|
|Date Deposited:||01 Jun 2015 12:40|
|Last Modified:||12 Aug 2015 05:41|
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