Goodhead, I, Capewell, P, Bailey, J, Beament, T, Chance, M, Kay, S, Forrester, S, MacLeod, A, Taylor, M and Noyes, H 2013, 'Whole-Genome sequencing of Trypanosoma brucei reveals introgression between subspecies that is associated with virulence' , mBio, 4 (4) , e00197.
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Human African trypanosomiasis is caused by two subspecies of Trypanosoma brucei. Trypanosoma brucei rhodesiense is found in East Africa and frequently causes acute disease, while Trypanosoma brucei gambiense is found in West Africa and is associated with chronic disease. Samples taken from a single focus of a Ugandan outbreak of T. b. rhodesiense in the 1980s were associated with either chronic or acute disease. We sequenced the whole genomes of two of these isolates, which showed that they are genetically distinct from each other. Analysis of single nucleotide polymorphism markers in a panel of 31 Ugandan isolates plus 32 controls revealed a mixture of East African and West African haplotypes, and some of these haplotypes were associated with the different virulence phenotypes. It has been shown recently that T. b. brucei and T. b. rhodesiense populations undergo genetic exchange in natural populations. Our analysis showed that these strains from the Ugandan epidemic were intermediate between the reference genome sequences of T. b. gambiense and T. b. brucei and contained haplotypes that were present in both subspecies. This suggests that the human-infective subspecies of T. brucei are not genetically isolated, and our data are consistent with genomic introgression between East African and West African T. b. brucei subspecies. This has implications for the control of the parasite, the spread of drug resistance, and understanding the variation in virulence and the emergence of human infectivity.
|Themes:||Subjects outside of the University Themes|
|Schools:||Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre|
|Journal or Publication Title:||mBio|
|Publisher:||American Society for Microbiology|
|Depositing User:||Dr Ian Goodhead|
|Date Deposited:||23 Sep 2015 16:59|
|Last Modified:||23 Sep 2015 16:59|
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