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Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei

Wen, Y, Lun, Z, Zhu, X, Hide, G and Lai, D 2016, 'Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei' , Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 41 , pp. 56-62.

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Abstract

The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
Publisher: Elsevier
ISSN: 1567-7257
Related URLs:
Funders: National Sciences Foundation of China, Guangzhou Science Technology and Innovation Commission, Natural Science Foundation of Guangdong Province, Fundamental Research Funds for the Central University
Depositing User: Professor Geoff Hide
Date Deposited: 06 Apr 2016 10:06
Last Modified: 11 Jul 2016 07:37
URI: http://usir.salford.ac.uk/id/eprint/38638

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