Faunce, CA, Paradies, HH and Quitschau, P 2003, 'Solution and structural properties of colloidal charged lipid A (diphosphate) dispersions' , The Journal of Physical Chemistry B, 107 (10) , pp. 2214-2227.

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Abstract

It has been possible to prepare electrostatically stabilized aqueous dispersions of lipid A (diphosphate) particles of low polydispersity at low ionic strength (1-10 mM NaCl) over a range of volume fractions of 1.5 × 10^-4 < < 5.75 × 10^-4 (25 C). These suspensions have been characterized by transmission electron microscopy, light scattering, osmotic pressure measurements, and small-angle X-ray scattering experiments at 25 C. All four measurements yielded independent values for particle sizes, weighted-average molecular weights, number-average molecular weights, and particle surface charge. The mean values obtained are = 37.59 ± 0.75 nm, = 24.89 ± 0.88 nm, = (10.55 ± 0.78) × 106 g/mol, = (9.81 ± 0.90) × 106 g/mol, and the effective surface charge Z* = (756 ± 85). Very good experimental agreement is found for the directly measured osmotic pressure values and those determined from light scattering and small-angle X-ray scattering measurements as a function of volume fraction, , by applying liquid-state theory models. Using the particle parameters for the lipid A (diphosphate) system as determined, the scattering functions and the osmotic pressures can be compared as a function of volume fraction with no adjustable parameters. The ordering of lipid A in solution revealed a body-centered cubic (bcc) type lattice (a = 36.14 nm) at volume fractions of 3.75 × 10^-4 < < 4.15 × 10^-4, whereas at volume fractions of 4.15 × 10^-4 < < 5.75 × 10^-4 in the presence of 1.0 mM NaCl a face-centered cubic (fcc) lattice type (a = 57.25 nm) was observed. Small-angle X-ray scattering experiments also indicate the presence of long-ranged order at 1.0 mM or at 10.0 mM NaCl for lipid A dispersions of 3.75 × 10^-4 < < 5.75 × 10^-4.

Item Type:	Article
Themes:	Subjects / Themes > Q Science > QD Chemistry Subjects / Themes > Q Science > QC Physics Subjects outside of the University Themes
Schools:	Colleges and Schools > College of Science & Technology Colleges and Schools > College of Science & Technology > School of Computing, Science and Engineering
Journal or Publication Title:	The Journal of Physical Chemistry B
Publisher:	American Chemical Society
Refereed:	Yes
ISSN:	15206106
Depositing User:	H Kenna
Date Deposited:	07 Sep 2007 09:50
Last Modified:	27 Sep 2011 12:28
URI:	http://usir.salford.ac.uk/id/eprint/398

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