Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge

Rattray, NJW, Zalloum, WA, Mansell, D, Latimer, J, Schwalbe, CH, Blake, AJ, Bichenkova, EV and Freeman, S 2012, 'Fluorescent probe for detection of bacteria : conformational trigger upon bacterial reduction of an azo bridge' , Chemical Communications, 48 , pp. 6393-6395.

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Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title: Chemical Communications
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Related URLs:
Funders: Unknown
Depositing User: J Latimer
Date Deposited: 24 Oct 2016 10:03
Last Modified: 21 Jul 2017 07:47
URI: http://usir.salford.ac.uk/id/eprint/39967

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