Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles

Miklavc, P, Ehinger, K, Sultan, A, Felder, T, Paul, P, Gottschalk, KE and Frick, M 2015, 'Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles' , Journal of Cell Science, 128 (6) , pp. 1193-1203.

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Abstract

In many secretory cells actin and myosin are specifically recruited to the surface of secretory granules following their fusion with the plasma membrane. Actomyosin-dependent compression of fused granules is essential to promote active extrusion of cargo. However, little is known about molecular mechanisms regulating actin coat formation and contraction. Here, we provide a detailed kinetic analysis of the molecules regulating actin coat contraction on fused lamellar bodies in primary alveolar type II cells. We demonstrate that ROCK1 and myosin light chain kinase 1 (MLCK1, also known as MYLK) translocate to fused lamellar bodies and activate myosin II on actin coats. However, myosin II activity is not sufficient for efficient actin coat contraction. In addition, cofilin-1 and α-actinin translocate to actin coats. ROCK1-dependent regulated actin depolymerisation by cofilin-1 in cooperation with actin crosslinking by α-actinin is essential for complete coat contraction. In summary, our data suggest a complementary role for regulated actin depolymerisation and crosslinking, and myosin II activity, to contract actin coats and drive secretion.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Journal of Cell Science
Publisher: Company of Biologists
ISSN: 0021-9533
Funders: Ministry of Science, Research and the Arts Baden-Wuerttemberg, Deutsche Forschungsgemeinschaft
Depositing User: P Miklavc
Date Deposited: 24 Oct 2016 10:47
Last Modified: 09 Aug 2017 06:50
URI: http://usir.salford.ac.uk/id/eprint/40254

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