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Mapping protein binding sites on the biomolecular corona of nanoparticles

Kelly, PM, Åberg, C, Polo, E, O'Connell, A, Cookman, J, Fallon, J, Krpetic, Z and Dawson, KA 2015, 'Mapping protein binding sites on the biomolecular corona of nanoparticles' , Nature Nanotechnology, 10 (5) , pp. 472-479.

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Abstract

Nanoparticles in a biological milieu are known to form a sufficiently long-lived and well-organized ‘corona’ of biomolecules to confer a biological identity to the particle. Because this nanoparticle–biomolecule complex interacts with cells and biological barriers, potentially engaging with different biological pathways, it is important to clarify the presentation of functional biomolecular motifs at its interface. Here, we demonstrate that by using antibody-labelled gold nanoparticles, differential centrifugal sedimentation and various imaging techniques it is possible to identify the spatial location of proteins, their functional motifs and their binding sites. We show that for transferrin-coated polystyrene nanoparticles only a minority of adsorbed proteins exhibit functional motifs and the spatial organization appears random, which is consistent, overall, with a stochastic and irreversible adsorption process. Our methods are applicable to a wide array of nanoparticles and can offer a microscopic molecular description of the biological identity of nanoparticles.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Nature Nanotechnology
Publisher: Macmillan
ISSN: 1748-3387
Related URLs:
Funders: Science Foundation Ireland (SFI), Irish Research Council, European Union Seventh Framework Programme, European Union Seventh Framework Programme, European Union Seventh Framework Programme
Depositing User: Dr Zeljka Krpetic
Date Deposited: 06 Oct 2016 13:27
Last Modified: 06 Oct 2016 13:27
URI: http://usir.salford.ac.uk/id/eprint/40287

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