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Revealing DNA interactions with exogenous agents by surface-enhanced raman scattering

Masetti, M, Xie, H, Krpetić, Z, Recanatini, M, Alvarez-Puebla, RA and Guerrini, L 2015, 'Revealing DNA interactions with exogenous agents by surface-enhanced raman scattering' , Journal of the American Chemical Society, 137 (1) , pp. 469-476.

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The standard protocols for DNA analysis largely involve polymerase chain reaction (PCR). However, DNA structures bound to chemical agents cannot be PCR-amplified, and therefore any sequence changes induced by external agents may be neglected. Thus, the development of analytical tools capable of characterizing the biochemical mechanisms associated with chemically induced DNA damage is demanded for the rational design of more effective chemotherapy drugs, understanding the mode of actions of carcinogenic chemicals, and monitoring the genotypic toxicology of environments. Here we report a fast, high-throughput, low-cost method for the characterization and quantitative recognition of DNA interactions with exogenous agents based on surface-enhanced Raman scattering spectroscopy. As representative chemical agents, we selected a chemotherapeutic drug (cisplatin) which forms covalent adducts with DNA, a duplex intercalating agent (methylene blue), and a cytotoxic metal ion (HgII) which inserts into T:T mismatches. Rich structural information on the DNA complex architecture and properties is provided by the unique changes of their SERS spectra, which also offer an efficient analytical tool to quantify the extent of such binding.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Journal of the American Chemical Society
Publisher: American Chemical Society
ISSN: 0002-7863
Related URLs:
Funders: European Research Council, European Research Council, Spanish Ministerio de Economia y Competitividad
Depositing User: Dr Zeljka Krpetic
Date Deposited: 06 Oct 2016 15:03
Last Modified: 06 Oct 2016 15:03

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