Corticosteroid treatment is associated with increased filamentous fungal burden in allergic fungal disease

Fraczek, MG, Chishimba, L, Niven, RM, Bromley, M, Simpson, A, Smyth, LJC, Denning, DW and Bowyer, P 2017, 'Corticosteroid treatment is associated with increased filamentous fungal burden in allergic fungal disease' , The Journal of Allergy and Clinical Immunology .

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Abstract

Background
Allergic diseases caused by fungi are common. The best understood conditions are allergic bronchopulmonaryaspergillosis (ABPA) and severe asthma with fungal sensitisation (SAFS). Our knowledge of the fungal microbiome (mycobiome) is limited to a few studies involving healthy individuals, asthmatics and smokers. No study has yet examined the mycobiome in fungal lung disease.
Objectives
The main aim of this study was to determine the mycobiome in lungs of individuals with well characterised fungal disease. A secondary objective was to determine possible effects of treatment on the mycobiome.
Methods
After bronchoscopy, ITS1 DNA was amplified and sequenced and fungal load determined by RT-PCR. Clinical and treatment variables were correlated with the main species identified. ABPA (n=16), SAFS (n=16), severe asthma not sensitised to fungi, (n=9), mild asthma patients(n=7) and 10 healthy controls were studied.
Results
The mycobiome was highly varied with severe asthmatics carrying higher loads of fungus. Healthy individuals had low fungal loads, mostly poorly characterised Malasezziales.The most common fungus in asthmatics was Aspergillus fumigatus complex and this taxon accounted for the increased burden of fungus in the high level samples. Corticosteroid treatment was significantly associated with increased fungal load (p<0.01).
Conclusions
The mycobiome is highly variable. Highest loads of fungus are observed in severe asthmatics and the most common fungus is Aspergillus fumigatus complex. Individuals receiving steroid therapy had significantly higher levels of Aspergillus and total fungus in their BAL.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: The Journal of Allergy and Clinical Immunology
Publisher: Elsevier
ISSN: 0091-6749
Related URLs:
Funders: Medical Research Council (MRC), EU Framework 7
Depositing User: LJC Smyth
Date Deposited: 29 Nov 2017 12:19
Last Modified: 29 Nov 2017 15:38
URI: http://usir.salford.ac.uk/id/eprint/44473

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