The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo

Zhang, X, Hong, X-K, Li, S-J, Lai, D-H, Hide, G ORCID: 0000-0002-3608-0175, Lun, Z-R and Wen, Y-Z 2018, 'The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo' , Experimental parasitology, 184 , pp. 115-120.

[img] PDF (Authors Accepted Manuscript) - Accepted Version
Restricted to Repository staff only until 13 December 2018.

Download (1MB) | Request a copy
[img] PDF (Supplementary Table 1) - Supplemental Material
Restricted to Repository staff only until 13 December 2018.

Download (725kB) | Request a copy
[img] Microsoft Word (Authors Accepted Manuscript) - Accepted Version
Restricted to Repository staff only

Download (995kB) | Request a copy
[img] Microsoft Word (Supplementary Table 1) - Supplemental Material
Restricted to Repository staff only

Download (22kB) | Request a copy

Abstract

Trypanosoma musculi, a common blood flagellate found in mice, is similar in morphology and life cycle to the rat trypanosome T. lewisi. Both species belong to the subgenus Herpetosoma, and as T. lewisi has recently been shown to be a zoonotic pathogen, there is concern that T. musculi could also be potentially infective to humans. To test this hypothesis, a well-established method, the normal human serum (NHS) incubation test, was carried out which distinguishes human and non-human infective trypanosomes. We found that T. musculi could grow in 0.31% NHS in vitro, and even kept their infectivity to mice after incubation with 10% NHS for 24 h. In in vivo experiments, T. musculi were only slightly affected by NHS injection, confirming that it was less sensitive to the NHS than T. b. brucei, but more sensitive than T. lewisi. This resistance probably does not rely on a restricted uptake of ApoL-1. Due to this partial resistance, we cannot definitively confirm that T. musculi has the potential for infection to humans. As resistance is less than that of T. lewisi, our data suggest that it is unlikely to be a zoonotic pathogen although we would advise caution in the case of immunocompromised people such as AIDS and cancer patients.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title: Experimental parasitology
Publisher: Elsevier
ISSN: 1090-2449
Related URLs:
Depositing User: Professor Geoff Hide
Date Deposited: 26 Jan 2018 09:38
Last Modified: 17 Oct 2018 20:48
URI: http://usir.salford.ac.uk/id/eprint/45064

Actions (login required)

Edit record (repository staff only) Edit record (repository staff only)

Downloads

Downloads per month over past year