Skin swabs with FTA® cards as a dry storage source for amphibian DNA

Ward, A, Hide, G ORCID: 0000-0002-3608-0175 and Jehle, R 2018, 'Skin swabs with FTA® cards as a dry storage source for amphibian DNA' , Conservation Genetics Resources .

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Amphibians are the most endangered group of vertebrates, and conservation measures increasingly rely on information drawn from genetic markers. The present study explores skin swabs with Whatman FTA® cards as a method to retrieve PCR-amplifiable amphibian DNA. Swabs from ten adult great crested newts (Triturus cristatus) were used to compare FTA® card-based protocols with tissue sampling based on toe clips. PCR success rates were measured for seven microsatellite markers and one mtDNA marker (ND4) after 6 months of sample storage. We demonstrate that the merging of eight FTA® card punches from Qiagen-based DNA extraction always led to successful amplifications in at least one replicate, at an overall PCR success rate of 78%. The newly established protocol has the potential for wide application to future DNA-based amphibian studies.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title: Conservation Genetics Resources
Publisher: Springer
ISSN: 1877-7252
Related URLs:
Depositing User: R Jehle
Date Deposited: 05 Mar 2018 13:39
Last Modified: 05 Mar 2018 14:18

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