Eustace, A, Smyth, LJC, Mitchell, L, Williamson, K, Plumb, J and Singh, D 2010, 'Identification of cells expressing Interleukin-17A and F in the lungs of COPD patients' , Chest .
Full text not available from this repository. (Request a copy)Abstract
Background: Lymphocytes secrete IL-17A and IL-17F which enhance innate immune responses. IL-17 expression has not been studied in COPD small airways. The aim was to quantify IL-17A and IL-17F expression in the peripheral lung tissue of COPD patients compared to controls and to identify inflammatory cells that express IL-17. Methods: IL-17 expression was assessed using immunohistochemistry in peripheral lung tissue (18 COPD patients, 10 smokers and 10 non-smokers with normal lung function) and induced sputum (12 COPD patients and 6 non-smokers). Alveolar macrophages from 8 COPD patients, 8 smokers and 7 non-smokers were used for RT-PCR. Results: The numbers of inflammatory cells expressing IL-17A in the small airway subepithelium was higher in COPD patients compared to smokers (p=0.01) and non-smokers (p=0.02). IL-17A expression was higher than IL-17F in this region. IL-17A was expressed by lymphocytes, neutrophils and macrophages (confirmed by RT-PCR). The expression of IL-17F was greater than IL-17A in epithelial cells and lymphoid follicles, although there were no differences between subject groups Conclusion: Our findings indicate different roles for IL-17A and IL-17F in the pathogenesis of COPD; IL-17A plays a role in small airway subepithelial inflammation, while IL-17F appears to play a more prominent role within lymphoid follicles.
| Item Type: | Article |
|---|---|
| Themes: | Subjects / Themes > Q Science > QR Microbiology Subjects outside of the University Themes |
| Schools: | Schools > School of Environment and Life Sciences |
| Journal or Publication Title: | Chest |
| Publisher: | American College of Chest Physicians |
| Refereed: | Yes |
| ISSN: | 0012-3692 |
| Depositing User: | Users 29196 not found. |
| Date Deposited: | 22 Dec 2010 16:37 |
| Last Modified: | 27 Aug 2021 22:33 |
| URI: | https://usir.salford.ac.uk/id/eprint/12672 |
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