Konrad, R, Connor, M, Ferry, N ORCID: https://orcid.org/0000-0003-3728-4302, Gatehouse, A and Babendreier, D
2009,
'Impact of transgenic oilseed rape expressing oryzacystatin-1 (OC-1) and of insecticidal proteins on longevity and digestive enzymes of the solitary bee Osmia bicornis.'
, Journal of insect physiology, 55 (4)
, pp. 305-13.
![]() |
PDF
- Published Version
Restricted to Repository staff only Download (499kB) | Request a copy |
Abstract
The risk that insect-resistant transgenic plants may pose for solitary bees was assessed by determining longevity of adult Osmia bicornis (O. rufa) chronically exposed to transgenic oilseed rape expressing oryzacystatin-1 (OC-1) or to the purified insecticidal proteins recombinant rOC-1, Kunitz soybean trypsin inhibitor (SBTI), Galanthus nivalis agglutinin (GNA), or Bacillus thuringiensis toxin Cry1Ab dissolved in sugar solution (at 0.01 and 0.1%, w:v, Cry1Ab only at 0.01%). Compared to control bees, longevity was significantly reduced by SBTI and GNA at both concentrations and by rOC-1 at 0.1%, but not by Cry1Ab or rOC-1 at 0.01%. Longevity on the OC-1 oilseed rape was not significantly different from the control plants. The effects of SBTI and rOC-1 on longevity were investigated through characterization of the digestive proteinases of O. bicornis and analysis of the response in proteinase profiles to ingestion of these proteinase inhibitors. A relatively complex profile of at least four types of soluble proteolytic enzymes was identified. Serine proteinases were found to be predominant, with metallo and especially cysteine proteinases making a smaller albeit significant contribution. The compensatory response to in vivo enzyme inhibition was similar for SBTI and rOC-1 although less pronounced for rOC-1. It consisted of a non-specific overproduction of native proteinases, both sensitive and insensitive, and the induction of a novel aspartic proteinase.
Item Type: | Article |
---|---|
Themes: | Built and Human Environment |
Schools: | Schools > School of Environment and Life Sciences |
Journal or Publication Title: | Journal of insect physiology |
Publisher: | Elsevier |
Refereed: | Yes |
ISSN: | 1879-1611 |
Depositing User: | Dr Natalie Ferry |
Date Deposited: | 06 Oct 2011 13:20 |
Last Modified: | 16 Feb 2022 12:45 |
URI: | http://usir.salford.ac.uk/id/eprint/17948 |
Actions (login required)
![]() |
Edit record (repository staff only) |