Elkord, E and Burt, D 2011, 'Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells' , Scandinavian Journal Of Immunology, 74 (4) , pp. 419-422.
Full text not available from this repository. (Request a copy)Abstract
We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
| Item Type: | Article |
|---|---|
| Themes: | Subjects outside of the University Themes |
| Schools: | Schools > School of Environment and Life Sciences > Biomedical Research Centre |
| Journal or Publication Title: | Scandinavian Journal Of Immunology |
| Publisher: | Blackwell Publishing |
| Refereed: | Yes |
| ISSN: | 0300-9475 |
| Depositing User: | E Elkord |
| Date Deposited: | 11 Oct 2011 15:18 |
| Last Modified: | 30 Nov 2015 23:56 |
| URI: | http://usir.salford.ac.uk/id/eprint/18228 |
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