Daayana, S, Elkord, E, Winters, U and Pawlita, M 2010, 'Phase II trial of imiquimod and HPV therapeutic vaccination in patients with vulval intraepithelial neoplasia.' , British Journal of Cancer, 102 (7) , pp. 1129-1136.
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Abstract
Vulval intraepithelial neoplasia (VIN) is a premalignant condition, which is frequently associated with type HPV16 infection, and multifocal disease has high rates of surgical treatment failure. Methods: We report a phase II clinical trial of the topical immunomodulator, imiquimod, for 8 weeks, followed by 3 doses (weeks 10, 14 and 18) of therapeutic human papillomavirus (HPV) vaccination (TA-CIN, fusion protein HPV16 E6E7L2) in 19 women with VIN grades 2 and 3. Histology and HPV testing of biopsies were performed at weeks 0, 10, 20 and 52. Intralesional infiltration of T-cell subsets and lymphocyte proliferation for HPV systemic immune responses were also assessed. Results: Lesion response (complete regression of VIN on histology) was observed in 32% (6 out of 19) of women at week 10, increasing to 58% (11 out of 19) at week 20 and 63% (12 out of 19) at week 52. At this time, 36% (5 out of 14) of lesions showed HPV16 clearance and 79% (15 out of 19) of women were symptom free. At week 20, after treatment with imiquimod and vaccination, there was significantly increased local infiltration of CD8 and CD4 T cells in lesion responders; in contrast, non-responders (persistent VIN by histology) showed an increased density of T regulatory cells. After vaccination, only lesion responders had significantly increased lympho-proliferation to the HPV vaccine antigens. Conclusion: The therapeutic effect of treatment depends on the differential immune response of responders and non-responders with affect locally and systemically.
Item Type: | Article |
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Themes: | Health and Wellbeing |
Schools: | Schools > School of Environment and Life Sciences |
Journal or Publication Title: | British Journal of Cancer |
Publisher: | Nature Publishing Group |
Refereed: | Yes |
ISSN: | 0007-0920 |
Depositing User: | E Elkord |
Date Deposited: | 11 Oct 2011 15:55 |
Last Modified: | 16 Feb 2022 12:52 |
URI: | http://usir.salford.ac.uk/id/eprint/18233 |
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