Botchway, SW, Parker, AW, Bisby, RH and Crisostomo, AG 2008, 'Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation' , Microscopy Research and Technique, 71 (4) , pp. 267-273.
Full text not available from this repository. (Request a copy)Abstract
The real-time uptake of serotonin, a neurotransmitter, by rat leukaemia mast cell line RBL-2H3 and 5-hydroxytryptophan by Chinese hamster V79 cells has been studied by fluorescence lifetime imaging microscopy (FLIM), monitoring ultraviolet (340 nm) fluorescence induced by two-photon sub-picosecond 630 nm excitation. Comparison with two-photon excitation with 590 nm photons or by three-photon excitation at 740 nm (Williams et al, 1999) shows that the use of 630 nm excitation provides optimal signal intensity and lowered background from auto-fluorescence of other cellular components. In intact cells, we observe using FLIM three distinct fluorescence lifetimes of serotonin and 5-hydroxytryptophan according to location. The normal fluorescence lifetimes of both serotonin (3.8 ns) and 5-hydroxytryptophan (3.5 ns) in solution are reduced to ~2.5 ns immediately on uptake into the cell cytosol. The lifetime of internalized serotonin in RBL-2H3 cells is further reduced to ~2.0 ns when stored within secretory vesicles.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Serotonin; fluorescence; multiphoton; cellular; imaging; lifetime; microscopy; 5-hydroxytryptophan; FLIM |
| Themes: | Subjects / Themes > Q Science > QH Natural history > QH301 Biology Subjects outside of the University Themes |
| Schools: | Schools > School of Environment and Life Sciences Schools > School of Environment and Life Sciences > Biomedical Research Centre |
| Journal or Publication Title: | Microscopy Research and Technique |
| Publisher: | John Wiley & Sons |
| Refereed: | Yes |
| ISSN: | 1059910X |
| Funders: | Science and Technology Facilities Council (STFC) |
| Depositing User: | RH Bisby |
| Date Deposited: | 24 Jun 2009 11:01 |
| Last Modified: | 30 Nov 2015 23:59 |
| URI: | http://usir.salford.ac.uk/id/eprint/2137 |
Actions (login required)
 |
Edit record (repository staff only) |
Tools
Tools