Radiation effects on enzymes

Beamont, KPG 1973, Radiation effects on enzymes , PhD thesis, Salford : University of Salford.

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Michaelis-Menten and maximum velocity values have been obtained for the arvin-catalysed hydrolysis of fibrinogen, benzoyl arginine ethyl ester and toluene p-sulphonyl arginine methyl ester. Optimum conditions of temperature (50°) and pH (8.0) have been detemined for hydrolysis of the synthetic substrates. Enzymatic Hydrolysis of TAME is compet- ively inhibited by benzoyl arginamide. Toluene-o-sulphonyl fluoride and p-TSF, on the other hand, are non-competitive inhibitors. Rapid loss of enzymatic activity occurs after y-irradiation in argon and N20 saturated solutions. Thereare indications that e-aq. and OH radicals contribute to a similar extent towards inactivation. Gel Filtration of the irradiated enzyme reveals the production of several low molecular weight fagments. Extensive destruction of amino- acid residues occurs during irradiation in argon.

Item Type: Thesis (PhD)
Contributors: Power, DM (Supervisor)
Depositing User: Institutional Repository
Date Deposited: 03 Oct 2012 13:34
Last Modified: 04 Aug 2022 11:27
URI: http://usir.salford.ac.uk/id/eprint/26572

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