Use of competition dialysis in the discovery of G-quadruplex selective ligands

Ragazzon, PA and Chaires, J 2007, 'Use of competition dialysis in the discovery of G-quadruplex selective ligands' , Methods, 43 (4) , pp. 313-323.

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Abstract

G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand oreintations, to bimolecular dimeric structures, and finally to tetramolecular parallel-stranded structures. These diverse structures present numerous potential small molecule binding sites with distinctive properties. There is mounting evidence for important functional roles for G-quadruplex structures in biology. G-quadruplexes may participate in the maintenance of telomeres, in transcriptional regulation and, in mRNA, may act to modulate translation. G-quadruplexes thus represent an attractive target for new small-molecule therapeutic agents. Competition dialysis provides a useful tool for the discovery of small molecules that selectively recognize the unique structural features of G-quadruplexes. The principles and practice of the competition dialysis experiment are described here.

Item Type:	Article
Themes:	Health and Wellbeing
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	Methods
Publisher:	Elsevier
Refereed:	Yes
ISSN:	1046-2023
Related URLs:	Publication
Funders:	Brown Cancer Center
Depositing User:	PA Ragazzon
Date Deposited:	26 Jan 2015 13:51
Last Modified:	16 Feb 2022 16:04
URI:	https://usir.salford.ac.uk/id/eprint/33308

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