Infection by Toxoplasma gondii, a severe parasite in neonates and AIDS patients, causes impaired anion secretion in airway epithelia

Guo, H-M, Gao, J-M, Luo, Y-L, Wen, Y-Z, Zhang, Y-L, Hide, G ORCID: https://orcid.org/0000-0002-3608-0175, Zhou, W-L, Ayala, FJ and Lun, Z-R 2015, 'Infection by Toxoplasma gondii, a severe parasite in neonates and AIDS patients, causes impaired anion secretion in airway epithelia' , Proceedings of the National Academy of Sciences of the United States of America, 112 (14) , pp. 4435-40.

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Abstract

The airway epithelia initiate and modulate the inflammatory responses to various pathogens. The cystic fibrosis transmembrane conductance regulator-mediated Cl(-) secretion system plays a key role in mucociliary clearance of inhaled pathogens. We have explored the effects of Toxoplasma gondii, an opportunistic intracellular protozoan parasite, on Cl(-) secretion of the mouse tracheal epithelia. In this study, ATP-induced Cl(-) secretion indicated the presence of a biphasic short-circuit current (Isc) response, which was mediated by a Ca(2+)-activated Cl(-) channel (CaCC) and the cystic fibrosis transmembrane conductance regulator. However, the ATP-evoked Cl(-) secretion in T. gondii-infected mouse tracheal epithelia and the elevation of [Ca(2+)]i in T. gondii-infected human airway epithelial cells were suppressed. Quantitative reverse transcription-PCR revealed that the mRNA expression level of the P2Y2 receptor (P2Y2-R) increased significantly in T. gondii-infected mouse tracheal cells. This revealed the influence that pathological changes in P2Y2-R had on the downstream signal, suggesting that P2Y2-R was involved in the mechanism underlying T. gondii infection in airways. These results link T. gondii infection as well as other pathogen infections to Cl(-) secretion, via P2Y2-R, which may provide new insights for the treatment of pneumonia caused by pathogens including T. gondii.

Item Type:	Article
Themes:	Health and Wellbeing Subjects outside of the University Themes
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title:	Proceedings of the National Academy of Sciences of the United States of America
Publisher:	National Academy of Sciences
Refereed:	Yes
ISSN:	1091-6490
Related URLs:	Publication
Funders:	National Science Foundation of China, National Basic Research Programme of China
Depositing User:	Professor Geoff Hide
Date Deposited:	08 May 2015 12:46
Last Modified:	15 Feb 2022 19:08
URI:	https://usir.salford.ac.uk/id/eprint/34462

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