The effects of corticosteroids on cytokine production from asthma lung lymphocytes

Kaur, M, Reynolds, S, Smyth, LJC, Simpson, K, Hall, S and Singh, D 2014, 'The effects of corticosteroids on cytokine production from asthma lung lymphocytes' , International immunopharmacology, 23 (2) , pp. 581-4.

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BACKGROUND Lymphocytes play a central role in the pathophysiology of asthma. Corticosteroids have a limited effect in severe asthma and we hypothesise that lymphocytes play a central role in corticosteroid insensitivity. We investigated the effects of corticosteroids on cytokine production from lung lymphocytes obtained from patients with moderate severe asthma (MSA) compared to mild asthma (MA) and healthy non-smokers (HNS). METHODS Bronchoalveolar lavage (BAL) cells obtained by bronchoscopy from patients with MSA and MA (n = 11 and n = 14 respectively) and HNS (n = 7) were stimulated with CD2/3/28 beads to activate the lymphocytes, in the presence or absence of dexamethasone (0.01-1 μM). Supernatants were assayed for IL-2, IFNγ, IL-17, IL-13 and IL-10 production. RESULTS Dexamethasone caused variable inhibition of cytokines; 1 μM inhibited IL-10 and IL-17 by 50% or lower, while inhibition > 50% was observed for IL-2, IL-13 and IFNγ. The effect of dexamethasone on IL-13 production was reduced in MSA. CONCLUSION These findings suggest that the production of specific lymphocyte derived cytokines is poorly suppressed by corticosteroids in MSA, which may be responsible for persistent airway inflammation in these patients

Item Type: Article
Themes: Health and Wellbeing
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: International immunopharmacology
Publisher: Elsevier
Refereed: Yes
ISSN: 1878-1705
Funders: Non funded research
Depositing User: LJC Smyth
Date Deposited: 15 May 2015 11:20
Last Modified: 27 Aug 2021 23:16

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