Magnetic sentinel lymph node biopsy in a murine tumour model

Ahmed, M, Woo, T, Ohashi, K, Suzuki, T, Kaneko, A, Hoshino, A, Zada, A, Baker, RD ORCID: https://orcid.org/0000-0003-3555-3425, Douek, M, Kusakabe, M and Sekino, M 2015, 'Magnetic sentinel lymph node biopsy in a murine tumour model' , Nanomedicine: Nanotechnology, Biology and Medicine, 12 (4) , pp. 1045-1052.

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Abstract

The magnetic technique for sentinel node biopsy provides a radioisotope-free alternative for staging breast cancer. It requires refinement to reduce “residual iron content” at injection sites by maximising lymphatic uptake to prevent “void artefacts” on magnetic resonance imaging (MRI), which could adversely affect clinical use. The site and timing of injection of magnetic tracer was evaluated in a murine tumour model (right hind limb) in 24 wild type mice. Right-sided intratumoural and left sided subcutaneous injection of magnetic tracer and assessment of nodal iron uptake on MRI, surgical excision and histopathological grading at time frames up to 24 hours were performed. Rapid iron uptake on MRI, smaller “void artefacts”(P < 0.001) and a significant increase in iron content with time were identified in the subcutaneous injection group (r = 0.937; P < 0.001).Subcutaneous injection and increasing delay between tracer injection and surgery is beneficial for lymphatic iron uptake.

Item Type:	Article
Schools:	Schools > Salford Business School > Salford Business School Research Centre
Journal or Publication Title:	Nanomedicine: Nanotechnology, Biology and Medicine
Publisher:	Elsevier
ISSN:	1549-9634
Related URLs:	Publication
Funders:	European Society of Surgical Oncology (ESSO), Young Surgeons and Alumni Club (EYSAC) Research Fellowship (2014-15) provided travel support for MA to conduct this research at the University of Tokyo, Japan.
Depositing User:	Prof Rose Dawn Baker
Date Deposited:	13 Apr 2016 10:55
Last Modified:	28 Aug 2021 01:59
URI:	http://usir.salford.ac.uk/id/eprint/38734

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